Our study on Bacteroides iron piracy is now out in @pnas.org. We show how surface-exposed lipoproteins bind xenosiderophores with different iron-chelating chemistries and propose a mechanism for xenosiderophore import across the outer membrane. Lots of structures (mostly X-ray this time)!
www.science.org/doi/full/10....
ComEC structure is out!! 😍
Congratulations to the authors! That’s not an easy one. We have been trying for years to get that structure…
We failed and gave up.
Now online. Great collaboration with the Tidow lab @seegerlab and the @embl SAXS beamline.
Capturing transient states of heterodimeric ABC transporter TM287/288 by time-resolved small-angle X-ray scattering: Biophysical Journal www.cell.com/biophysj/ful...
2D classes of the 58 kDa sample collected on the 100 keV Tundra TEM with a Falcon C detector at @nusbf.bsky.social @newcastleuni.bsky.social. Still much work to do to optimise the sample & data collection but its cool to see what a 100 keV TEM is capable of. #cryoEM @recon4imd.bsky.social
Latest research from the lab out now. We examined the role and expression of fimbriae during UPEC bloodstream infection. A real team effort and brilliant collaboration with Mark Schembri’s lab in Brisbane. Thanks to the MRC and BBSRC for supporting our research @ukri.org
First paper for PhD student in the Bolam / Lowe labs Natalia Łoś on a the characterisation of a new CBM family that binds (beta) mannan! #glycotime @natalia-los.bsky.social 🥳🥳
link.springer.com/article/10.1...
65 years after Peter Mitchell’s chemiosmotic theory, we report an association between ATP synthase and the electron transport chain. In the preprint led by @longzhou88.bsky.social, we describe a large supercomplex and show how the supramolecular organization contribute to oxidative phosphorylation 🧵
Iron is an essential element that can be growth-limiting in microbial communities,
particularly those present within host organisms. To acquire iro...
Natural transformation is one of the major pathways of horizontal gene transfer in bacteria, enabling the acquisition of extracellular DNA and its integration into the host genome. ComEC is a membrane...
www.science.org
Excited to share this paper from @yiechanglin.bsky.social showing how lipids can move between bacterial membranes along the bridge like protein TamB. Also happy to be able to post the cool movie. 🎥🌟 #MDsimulations #StructuralBiology doi.org/10.1016/j.bp...
Happy to have this collaborative work with Zach Armstrong now out in Angewandte chemie doi.org/10.1002/anie... .
We use a linked assay, coupled with fluorescent probes, to analyse mucin active carbohydrate sulfatase kinetics, cellular location, and inhibition.
Augustinas Silale
Author summary Extraintestinal pathogenic Escherichia coli (ExPEC) colonise the human gut without causing disease but can disseminate to the urinary tract and bloodstream. Understanding the mechanisms...
β-mannans are plant structural and storage polysaccharides prevalent in the human diet. Their degradation in the gastrointestinal tract is mediated by the human gut microbiota (HGM) through expression...
Structures of the heterodimeric ATP-binding cassette (ABC) transporter TM287/288 have
previously been determined in several states indicating large conformational changes
during its reaction cycle. Ho...
We report plate-based coupled assays to rapidly and quantitatively determine the activity and selectivity of mucin-active carbohydrate sulfatases. This fluorogenic assay further enables inhibitor ide...
The mitochondrial oxidative phosphorylation system powering most aerobic organisms is typically depicted as two spatially segregated machineries(1): electron transport chain complexes I-IV (ETC CI-CIV
