High-accuracy hierarchical rRNA operon profiling resolves genomovar-level taxonomy and microdiversity using Nanopore sequencing.
www.biorxiv.org/content/10.6...
For many years you needed UMIs and similar dark molecular biology magic to get perfect amplicon sequences from @nanoporetech.com data. With recent accuracy improvements+really clever algorithms that is now changed. Savont unlocks ASVs from low coverage nanopore amplicon data 🤯🤯🤯
Morten Kam Dahl Dueholm
Come to ONT's industry event at ASM to hear about two exciting new tools from @jimshaw.bsky.social! SNPmers (polymorphic kmers) are leveraged for impressive performance in both myloasm (metagenomic assembly) and savont (16S ASVs). Registration link 👇
www.biorxiv.org
Rasmus Kirkegaard
Our method, savont, for generating amplicon sequence variants (ASVs) for long-read amplicons is now on bioRxiv.
Work with @lh3lh3.bsky.social and help from @mkddueholm.bsky.social and team (Marie Riisgaard-Jensen, @kirk3gaard.bsky.social, Kasper Skytte Andersen)
github.com/bluenote-157...
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Jim Shaw
Christine He
Have a look at our new paper on sulfur metabolism in cable bacteria, first author Lea Plum-Jensen: academic.oup.com/ismej/advanc...
It's an interesting case of a pathway gap being filled using AlphaFold and subsequently verified biochemically. @ismepublications.bsky.social
Join Jim Shaw at #ASMicrobe to uncover how to unlock high-resolution, strain-level microbiome insights. Learn how this is could be crucial to helping deliver more precise diagnostics and treatment in the future. https://bit.ly/4v4XuKE
Sensitive long-read amplicon sequence variant recovery with savont https://www.biorxiv.org/content/10.64898/2026.05.26.727271v1
Sensitive long-read amplicon sequence variant recovery with savont https://www.biorxiv.org/content/10.64898/2026.05.26.727271v1