Fully manual 96-well protocol is also at protocols.io: www.protocols.io/view/tirtl-s.... Requires only manual multichannel pipettes, no specialised equipment! (3/4)
Code is on the Github github.com/pogorely/TIRTL, and there is a new R package with more TIRTL-seq data analysis and visualization functions (actively developed by @nick-clark-bioinfo.bsky.social), early version is ready for testing github.com/NicholasClar...! Congrats to TIRTL team!(4/4)
Detailed 384-well protocol is at protocols.io: www.protocols.io/view/tirtl-s... (2/4)
Excited to see our TIRTL-seq method published in Nature Methods www.nature.com/articles/s41...! Some updates after the revision: (1/4)
Mikhail Pogorelyy
Two complementary studies from our group out today: a therapeutic peptide vaccine trial for fibrolamellar carcinoma (FLC) & TIRTL-Seq, our robust method for deep quantitative paired TCR sequencing a🧵w/ @markyarchoan.bsky.social & @pogorely.bsky.social www.nature.com/articles/s41...
