Last Saturday morning ☕ saved for this gem:
www.science.org/doi/10.1126/...
CRISPR screening reveals TRIM25 as a pH-sensitive sensor that specifically targets foreign RNAs entering through endosomes. N1-methylpseudouridine helps repel TRIM25 binding & enhances protein production.
Exogenous messenger RNAs (mRNAs) require cellular machinery for delivery and translation but also encounter inhibitory factors. To investigate their regulation, we performed genome-wide CRISPR screens...