Another example of spurious effects when you try to interpret RNA biology from DNA introduced into cells without confirming exactly what RNA processing is happening. Important and common confounder - great paper!
Stephen Floor
Our collaboration with Tony Mustoe's lab us out today. MPRA libraries and possible cryptic splicing in MPRA reporters, or reporters in general. The additional novelty here is that such events are controlled or influenced by AU-rich sequences.
www.nature.com/articles/s41...
Massively parallel reporter assays (MPRAs) are powerful technologies for measuring the impact of non-coding sequences on gene expression. Here, the authors demonstrate that MPRA reporters often underg...
