RNA-guided endonucleases have rapidly advanced as powerful tools in the field of genome editing, enabling highly precise and targeted genome modifications. The expansion of the genome editing toolbox has been largely driven by CRISPR-Cas systems. However, over the past few years, transposon-derived nucleases from both prokaryotic and eukaryotic systems (identified as evolutionary ancestors of some Cas proteins) have emerged as a new genome editing tool.1,2 TnpB is a family of RNA-guided endonucleases encoded within prokaryotic transposons IS200/605 and IS607 and has evolved into a type-V CRISPR-Cas effector, i.e., Cas12.
