Assistant Professor at UMich, PhD at MIT,
NIH Director's New Innovator Awardee,
MIT Technology Review's Innovator Under 35,
www.spatialbiodynamics.org
Changyang Linghu
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GLOBE provides a versatile framework to dissect physiological and pathological cellular processes at brain-wide scale. This work is led by Dr. Dongqing Shi in our lab and driven by a cross-disciplinary effort from our team and collaborators. (6/6)
Dataset: zenodo.org/records/20241204
Introducing GLOBE—a new technology for brain-wide, single-cell recording of cellular activity in vivo. GLOBE can continuously record from nearly a quarter million individual neurons simultaneously across a mouse brain.
Preprint out on bioRxiv: doi.org/10.64898/202...
🧵(1/6)
To understand complex brain functions, we need to see deep, distributed networks working together. But live measurement often forces a tough choice: zoom in on local cells, or zoom out and lose cellular resolution. GLOBE eliminates this tradeoff via protein tape recorders. (2/6)
Scale: 219,703 neurons recorded simultaneously in a mouse brain in vivo with spatiotemporal resolution
Sensitivity: resolves behaviorally relevant Fos transcriptional activity continuously across 5.5 days
Precision: 3.1–6.7 hours timestamp precision (median absolute error) (3/6)
How it works:
1️⃣ Protein tape recorders log analog signal amplitudes along a continuous time axis in a cell.
2️⃣ Post-mortem scalable readout requires only standard light microscopy (no exotic hardware needed).
3️⃣ It is compatible with expansion microscopy and RNA readouts. (4/6)
GLOBE resolves brain-wide spatiotemporal structure of neuronal activity in memory encoding and retrieval, revealing heterogeneous distribution of distinct temporal modes of Fos transcriptional dynamics. Stability of the structure scales up with more sampled cells per brain. (5/6)
