Now out in Nature! We visualize infection of the RNA virus RSV in real-time with single-vRNP resolution to understand how RSV establishes viral factories, biomolecular condensates that act as sites of viral replication. A huge collaborative effort led by Dhanushika Ratnayake!
rdcu.be/e1bBW
The eTIS helps predict translation start site efficiency in any mRNA and aids in mRNA sequence optimization for vaccine mRNAs and production of therapeutic proteins.
This was a highly collaborative and multidisciplinary project, involving massively-parallel reporter assays, bioinformatics, machine learning, single-molecule imaging and cryo-EM.
Marvin Tanenbaum
Also check out this more detailed description of the work by lead author Bram Verhagen!
For 40 years the 8-nt ‘Kozak Sequence’ was thought to mark sites of translation initiation. In a new study, we revise this model by identifying an ~80-nt sequence—the extended Translation Initiation Sequence (eTIS)— that guides ribosomes to correct start sites. 🧵
www.biorxiv.org/content/10.6...
An amazing collaboration with David Liedtke and @niels-fischer.bsky.social; @luciabarmar.bsky.social and Jeroen de Ridder; Carlos Alvarado and Jody Puglisi; @michamuller.bsky.social; Michal Swirski and @edv.bsky.social
A fantastic effort by first authors Huib Rabouw, @janinschoko.bsky.social, @michamuller.bsky.social with lots of help in image analysis by @baarsmatthijs.bsky.social and @jakobpueschel.bsky.social. Also a great collaboration with the groups of @hansclevers.bsky.social and Ron Fouchier!
Out now in Cell Systems: We develop a technique to visualize influenza A virus in live cells with single viral RNA resolution. Combined with precise readouts of viral transcription, we map the whole influenza A viral life cycle and identify key bottlenecks in infection.
www.cell.com/cell-systems...
Marvin Tanenbaum
Marvin Tanenbaum
www.biorxiv.org
Excited to share our new paper! We developed a method to visualize proteasomal degradation at the single–molecule level in live cells, enabling us to dissect distinct modes of substrate engagement, probe co-factor dependence, and study proteasome–ribosome collisions.
www.biorxiv.org/content/10.6...
Marvin Tanenbaum
Marvin Tanenbaum
In this study, an imaging technology, “VISUN,” is developed that allows live-cell
visualization of unmodified influenza virus during infection with single-vRNP resolution.
Combining VISUN with multipl...
Nature - Viral ribonucleoprotein–viral protein networks form pre-replication centres that nucleate viral factories and drive respiratory syncytial virus replication.
