Still obsessed with histidine (and its mimics)! Our latest work from Benjamin 🎉 uses genetic code expansion—tuning the primary coordination sphere of a dizinc metallohydrolase—lowering the catalytic pKa and boosting activity up to 5x at acidic pH. Check it out! doi.org/10.1021/acs.biochem.5c00768
doi.org
Hydrolytic metalloenzymes employ Lewis-acidic metal cofactors to activate water molecules, generating nucleophilic hydroxide species that facilitate catalysis. Their catalytic efficiency across a wide...
