📣Publication alert: Julian Stingele and his team show that the chemotherapeutic drug azacitidine damages RNA and reveal a possible approach to improve therapies🤩👏
www.sciencedirect.com/science/arti...
@jstingele.bsky.social @lmu.de @shubozhao.bsky.social
Proud to share our latest preprint! 🥳
Using a multidisciplinary approach, we uncover a dual function of the snR37 H/ACA snoRNP - combining pseudouridylation with ribosome chaperone activity within a single RNP.🔬🧬
Challenging the traditional view of snoRNP function. 👇
#cryoEM #ribosome #RNA #snoRNA
Excessive RNA damage activates cellular stress responses, triggering cell death. However, pathways that negatively regulate RNA damage responses are l…
On that topic, also check out the recent publication by the Deuerling, Shu-oh Shan and Ban labs
Only in this collision geometry do the positioning of the two RACK1s allow ZAK’s RACK1-interacting motif (RIM) to engage them and enabling its SAM domains to dimerize — the key step for activation. This activation is further negatively regulated by SERBP1 competing for the RACK1 interface. 4/4
Despite being the driving force behind the RSR, the interaction between ZAK and ribosomes has remained elusive for years. Here, we show how ZAK is recruited to ribosomes in native as well as stress-induced conditions, and how collision-specific interactions organized on RACK1 mediate activation. 2/4
ZAK is constitutively recruited to ribosomes via its RACK1-interacting helix (RIH), the eS27-pin, and the ES7-contacting patch. Upon stress, ribosome collisions generate a unique interface that enables specific contacts — the ES6-patch and the RIH-peptide. 3/4
How are histones acetylated as they emerge from the ribosome? We show that NAA40 cooperates with the NAC complex to enable co-translational acetylation of H2A/H4, revealing NAC as a coordinator of nascent protein modification. Read more about our collaboration with @kirmizislab.bsky.social #cryoEM
Gene Center Munich @LMU Munich
A true collaborative effort from the Pertschy, Henras, Woodson & Beckmann labs (@erc.europa.eu), together with many fantastic collaborators.
@uni-graz.at @cbitoulouse.bsky.social @woodsonlab.bsky.social @genecenter-lmu.bsky.social @lmu.de
#UniGraz #CNRS #JohnsHopkins #LMU #ERCSyG
How does the kinase ZAK sense ribosome collisions? Find out in our latest collaboration with the @greenlab.bsky.social @doubleshuang.bsky.social @Vienna Huso: 1/4
rdcu.be/eRmJl
#ribosome #cryoEM #LMU #JHMI
Beckmann Lab
This is how co-translational N-terminal myristoylation occurs on the ribosome. NMT1 acts together with NAC, but after the release of MetAP. Cryo-EM study by Timo Denk et al. @beckmannlab.bsky.social @giglionelab.bsky.social www.nature.com/articles/s41...
