Genomics & qPCR surveillance of soils, sludge & sewage 🏞️💩😷 | RNA viruses, AMR genes & mobile elements in environmental microbiomes | Research Fellow @bangor.ac.uk | Building the evidence base for wastewater-informed public health in Wales 🏴
Luke Hillary
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DNase treating your viromes? Unlike soil viromes, this looks like it might not have as big an effect in stool (at least in our study).
🧵 3/5
Firing up the MDA kit? We got STRONG compositional distortions and reduced richness. Whilst this effect is generally known, MDA continues to be used. Filtering out ssDNA viruses partially compensated for this, but at a cost in downstream data analyses.
🧵 2/5