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Prime Assembly integrates twinPE with a Gibson-like strategy to assemble multiple overlapping DNA templates within cells for large DNA insertion of up to 11 kb #NBThighlight www.nature.com/articles/s41...
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Prime assembly can initiate Gibson-like assembly in cells to generate gene insertions without double-stranded DNA breaks, recombinases or homology-directed repair.
www.nature.com
Prime assembly with linear DNA donors enables large genomic insertions - Nature
Nature Biotechnology