💙 bluesky research 💙 reproducible science is excellent science. Editor @NatureProtocols / I have views and they’re mine!
Currently based in China
Rosy Favicchio
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The procedure covers the synthesis and purification of the ionizable lipid excipient, the preparation of mRNA LNPs, LNP transfection to placental trophoblast cells and the biodistribution and mRNA expression in the placentas of time-dated pregnant mice.
Strain-promoted azide–alkyne cycloaddition (SPAAC) chemistry is used to covalently conjugate dibenzocyclooctyne (DBCO)-labeled antibodies or antibody fragments to LNPs containing azide.
This workflow permits simultaneous profiling of chromatin accessibility and gene expression in single nuclei from tens to hundreds of samples and up to 1 million nuclei in a single run and is suitable for a wide variety of sample types.
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This protocol offers a versatile approach to the precise targeting of nucleic acid-containing lipid nanoparticles to cell types or tissues, such as liver- and lung-targeting LNPs in vivo and ex vivo human primary T cell-targeting LNPs.
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A protocol for the preparation and evaluation of placenta-tropic mRNA LNPs for treating pregnancy disorders.
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This protocol covers brain MRI and image segmentation for anatomical modeling and EEG signal reconstruction, validation of the oscillatory signals, oscillatory phase targeting accuracy and the main experiment of phase-coupled real-time EEG– TMS.
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This mRNA LNP formulation presented is effective for treating pre-eclampsia in mouse models of the condition and could be expanded for the treatment of other pregnancy disorders.
Readcube link: rdcu.be/e7O8o
Real-time EEG–TMS enables the timing of TMS pulses relative to target oscillatory brain states with millisecond-precision
@paolobelardinelli.bsky.social
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Single-cell ultra-high-throughput multiplexed sequencing leverages a combinatorial indexing strategy in which chromatin and mRNA are indexed in situ using sample-specific barcodes, and encapsulated into microfluidic droplets. @tommytang.bsky.social
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After antibody labeling and LNP conjugation, tLNPs are purified by size exclusion chromatography and characterized ex vivo and in vivo.
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