For many years you needed UMIs and similar dark molecular biology magic to get perfect amplicon sequences from @nanoporetech.com data. With recent accuracy improvements+really clever algorithms that is now changed. Savont unlocks ASVs from low coverage nanopore amplicon data 🤯🤯🤯
When you run out of slots on the P24 it is handy to have a bunch of P2 solos 🟩🟩🧬 @nanoporetech.com
Is @nanoporetech.com hac v6 better than 5.2.0 sup? The short answer is no. But are a few errors in a genome worth 5 times more basecalling compute?
github.com/Kirk3gaard/M...
Abstract submissions for the T2T Face-to-Face conference are open through June 15th!
Join the community Sept. 3–4 in Santa Cruz, CA, for two days of discussion and collaboration around telomere-to-telomere genomics and pangenomics.
Learn more: sites.google.com/ucsc.edu/t2t...
Without trusted references, methylation data is hard to interpret.
Using ATCC Genome Portal resources and nanopore sequencing, this webinar shows how to turn raw microbial modification signals into biological insight. https://bit.ly/4o369uU
Thought i'd highlight that the ONT London Calling tech talk is now up. Points of interest for the microbiome community:
1) Direct RNA multiplexing now available. Can now run 24 samples per flow cell, recover full-length transcripts with 8 base pair modifications...
www.youtube.com/watch?v=CE69...
Hac 6.0.0 seems like a mixed bag to me compared to sup 5.2.0 based on github.com/Kirk3gaard/M... . Thanks for your hard work @kirk3gaard.bsky.social
Jeremy Wang developed rammap, a minimap2 rewrite in Rust. It achieves comparable or better performance than minimap2 and produces identical output to minimap2. During rewrite, Jeremy found two long-existing bugs in minimap2 which are fixed in v2.31. www.biorxiv.org/content/10.6...
New blog post!
I analyse the new [email protected] basecalling model from @nanoporetech.com and discuss the conspicuous lack of a new sup model:
rrwick.github.io/2026/06/11/d...
It's also a boon for service providers with proms and high throughput. Many users can't/don't rebasecall and running sup on-machine limited the # of flow cells that could be run. v6 hac model means that more flow cells can be run concurrently to provide Q23 data directly for clients to run with.
