****2-NBDG does not measure glucose uptake****
Just your regular reminder of this fact 😁.
See the rigorous proof of this fact in @lindavsinclair.bsky.social 2020 paper.
This paper was 6 years ago!! Stop using 2NBDG to measure glucose uptake already 🙄
pmc.ncbi.nlm.nih.gov/articles/PMC...
pmc.ncbi.nlm.nih.gov
Assays to monitor the metabolic state or nutrient uptake capacity of immune cells at a single cell level are increasingly in demand. One assay, used by many immunologists, employs 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose ...