Lab of Petr Broz @ UNIL. Cell death, innate immunology and pathogen defense
Broz Lab
Loading...
You can hear all about this in a few weeks in Canada where @ehartenian.bsky.social will be presenting it @keystonesymposia.bsky.social on Innate Immunity
Congratulations to Jakub who led this project and all authors for their contributions!
Fragmentation of these membranes increases caspase-4 binding to LPS, and its caspase activity, which is an essential part of inflammasome activation.
Finally, we show that increased membrane curvature makes more sites for caspase4 to bind, which we observe with immunogold/TEM and MD simulations, thanks to key contributions from @masarykuniversity.bsky.social
With key contributions from @leonieanton.bsky.social performing cryo-CLEM, we were able to visualize deformation of Salmonella membranes on GBP1 coated cytosolic Salmonella, but not vacuolar Salmonella.
We’re happy to share our updated manuscript on Ninj1, incorporating measurements of membrane tension, cellular assays of ninj1 and ninj2 dimer stability and MD simulations of ninj1 oligmers opening and forming (see below!). @biorxivpreprint.bsky.social
www.biorxiv.org/content/10.1...
Finishing up in snowy Banff with current and former Broz Lab members after the innate immunity symposium. @jakubbegan.bsky.social @kaiwenchen-lab.bsky.social @ehartenian.bsky.social @keystonesymposia.bsky.social
Broz Lab
We highlight the key role of GBP1 as a mechanoenzyme, capable of fragmenting LPS in vitro, dependent on the GTP (check out those mini micelles in the TEM on the bottom right!).
We're thrilled to share the lab's latest publication demonstrating how caspase-4 and the non canonical inflammasome recognize cytosolic LPS by preferentially binding to the convex portions of membranes - both bacterial and micelles @cp-immunity.bsky.social
www.sciencedirect.com/science/arti...
Broz Lab
Congratulations to @jakubegan.bsky.social, @brozlab.bsky.social et al. A nice reminder that lipid acyl chains are not just defining binding interactions. They tune membrane curvature and flexibility, adding an additional layer of regulation to protein activity and cellular signalling
