Everyone in our field needs to stop what they are doing and read this magnificent work of ART & KNOWLEDGE that just dropped!
Sarah
In this study, we used Cas12a to disrupt >27,000 combinations of 233 DNA damage response genes in MCF10A and RPE-1 p53 KO cells, monitoring changes in cell viability. From this analysis, we identified over 750 high-confidence positive (buffering) or negative (synthetic lethal/sick) interactions.
Additionally, we identified robust suppressor interactions between the DCLRE1B gene encoding the nuclease APOLLO and the NHEJ genes XRCC4, LIG4, and NHEJ1.
Our full dataset can be queried at ddr-genetic-interactions.ciccialab-database.com
This has been an 8-year journey, and we hope that our studies will be useful to the community!
This work wouldn't have been possible without @angelotaglialatela.bsky.social, Jen-Wei Huang, @giuseppeleuzzi.bsky.social, and members of the labs of @traver.bsky.social, Raphael Guerois, @labvincenzo.bsky.social, Raul Rabadan, @nussenza.bsky.social, @jenscs83.bsky.social, @heyzajr.bsky.social.
Another classic from @albertociccia.bsky.social & @angelotaglialatela.bsky.social!
We uncovered synthetic lethal interactions between Fanconi anemia genes and GEN1, CIP2A, and RHNO1; the translesion polymerase REV1-Pol ζ and the MCM8/9-HROB DNA helicase; and the DNA translocase SMARCAL1 and the FANCM complex, as reported also by @durocher1.bsky.social and @jcornlab.bsky.social.
Alberto Ciccia
Excited to finally share our preprint on mapping the genetic interaction network of the DNA damage response with combinatorial knockout screens led by Sam Hayward, Alina Vaitsiankova, and Tomas Lama-Diaz! www.biorxiv.org/cgi/content/...
Happy to share our new review in @annualreviews.bsky.social on abasic sites and their impact on DNA replication written by @angelotaglialatela.bsky.social.
www.annualreviews.org/content/jour...
Alberto Ciccia
Alberto Ciccia
Alberto Ciccia
Alberto Ciccia
The DNA damage response (DDR) is a complex network of cellular pathways that ensures the faithful maintenance of our genomes upon a wide array of genomic insults. To elucidate the functional architect...
www.biorxiv.org
Apurinic/apyrimidinic (AP) sites, also known as abasic sites, are among the most frequent DNA lesions, arising spontaneously or as intermediates in base excision repair. Their structural impediment to...
Are you ready for the Summer? Social DNAing is! Check out the 2026 Summer line-up! Please note one schedule change on June 4th, 2026. Please RT! Sign up at cancer.columbia.edu/research/pro...
