Researchers in the Department of Molecular Cell Biology and Immunology (MCBI) perform fundamental and preclinical research in the field of Cancer, Neuro and Mucosal Immunology at the
Amsterdam UMC.
Molecular Cell Biology and Immunology Lab
Congratulations to MCBI postdoc Sofia Ibáñez-Molero (and co-authors) for publishing in @nature.com her cluster method developed during her PhD at @nkinl.bsky.social in @peeperlab.bsky.social. By always excluding doublets in glow cytometry you might exclude some gems.. www.nature.com/articles/s41...
Molecular Cell Biology and Immunology Lab
Our lab is hiring a PhD student to investigate how glycans affect antitumor immune responses in metastases and how this can be modulated to improve immunotherapy! More info here 👇
#glycotime
werkenbij.amsterdamumc.org/en/vacatures...
Siglecs are of particular relevance for immune evasion by cancer cells, and although they differ between human and mice, mouse Siglec-E is thought to be the closest homologue to human Siglec-7 and -9. Magali and team decided to explore the expression of Siglec-E and its ligands in a melanoma model.
New work from the department! In this work, Magali and team characterized the presence sialic acid-containing glycans in the widely-used B16OVA melanoma model and the expression of Siglec-E, an immunosuppressive receptor able to bind to sialic acid.
academic.oup.com/glycob/artic...
They found that in B16OVA, sialylated O-glycans represent the main source of Siglec-E ligands. In the tumor microenvironment (TME), macrophages, neutrophils, and CD8 but not CD4 T cells expressed Siglec-E, suggesting that these could be the main targets of Siglec-E-mediated immunosuppression.
Molecular Cell Biology and Immunology Lab
On the 1st day of the @cancercenterams.bsky.social annual conference Lotte De Winde presented her recently published data on the development of lymphoma-on-a-chip model to study the role of fibroblasts in this pathology! You can find the publication here doi.org/10.1016/j.mt...
Congratulations to @magalicocci.bsky.social @fabriziochiodo.bsky.social and all other co-authors!
Importantly, Siglec-E expression (and co-expression with PD-1) on T cell subsets varied across tissues, showing different levels in the TME, spleen, blood, and tumor-draining lymph nodes, highlighting the relevance of considering the tissular context when exploring the function of this receptor.
Interestingly, Siglec-E seemed to be mostly expressed by activated effector memory T cells with low levels of PD-1, indicating that Siglec-E could dampens the activation of these cells despite their low PD-1 expression.
