Using fiber photometry, mesoscopic imaging, and two-photon microscopy, we simultaneously monitored ACh together with DA, 5-HT, NE, and calcium signals with high spatiotemporal resolution.
www.nature.com/articles/s41... (3/3)
(1/3)Excited to share PKU tags—our lab’s latest contribution expanding the toolkit for cell-type labeling with a new shape dimension! Developed at PKU, these genetically encoded tools enable multiplex cell-type labeling compatible with both light and electron microscopy.
Excited to share our first red ACh sensor! 🔴
We developed GRAB_rACh1h, the first genetically encoded red fluorescent sensor for recording acetylcholine dynamics in vivo. (1/3)
Compared with existing ACh sensors, rACh1h exhibits larger responses (5-fold ΔF/F0 relative to gACh3.0), larger SNR and higher affinity. Most importantly, its red-shifted spectrum enables multiplex recording with green fluorescent sensors. (2/3)
Glad to see Yu Zheng PhD @zheng-yu.bsky.social highlighting the far-red dopamine sensor for multiplex imaging of in vivo neuromodulation. Happy to share these probes to the community.
Having trouble getting the probes or dyes? Contact us at [email protected] — we will be more than happy to help.
BioRxiv link
www.biorxiv.org/cgi/content/...
We’re bringing our latest progress in neuromodulator imaging to #SfN2025! Don’t miss Dr. Li’s talk (Nov 19) and our lab’s posters (Nov 16). We look forward to connecting, exchanging ideas, and exploring collaborations. #neuroscience #neuroimaging #biosensors @sfn.org
(3/3)Moreover, we showcased the power of PKU tags for in vivo shape-based “barcoding’’ of multiple cell types, delivering high-contrast and easily distinguishable signals in both light and electron microscopy.
(2/3)By integrating self-assembling protein polymers, spectrally distinct fluorescent proteins, and a nuclear targeting motif, PKU tags provide a versatile palette of cell-labeling tools. With combinatorial strategy, the system can theoretically scale to hundreds of patterns.