Assistant Professor at Penn State University.
Looking at cool tiny complex things through electromagnetic lens.
Today, chromatin remodeling. Tomorrow? Who knows.
Opinions my own
Jean-Paul Armache
A wonderful collaboration between Jess Tyler lab, @epicypher.bsky.social, @gcloner.bsky.social, James Kadonaga and our lab at PSU. In this article, we provide the significance of the nucleosome acidic patch.
doi.org/10.64898/202...
doi.org
Jean-Paul Armache
This was a highly collaborative work between multiple labs, performed by a talented grad student in the Armache and Murakami labs, Natalie Smith. Thank you to all the authors for their incredible work!
We did a thing... We purified native transcriptional complexes from Drosophila embryos, obtaining +/- stalk Pol II elongation complexes, native nucleosomes, as well as a nucleosome elongation complex. For more details please check out: doi.org/10.1101/2025...
Our laboratory contributed to this work by resolving acidic patch-binding nanobodies developed by @epicypher.bsky.social. I’d like to thank our wonderful collaborators, and Emma Saccone (@emmasaccone.bsky.social)(data analysis, interprt., figs) and Heather Folkwein (@hjf3.bsky.social)(sample prep)
New collaborative paper between JPArmache and Bowman (@bowmanlab-jhu.bsky.social) labs show how the yeast CHD1 chromatin remodeler depends on activator elements to distort nucleosomal DNA. This explains how the NegC inhibitor blocks activity. www.nature.com/articles/s41...
We coupled in depth mutagenesis and biochemistry with high-resolution cryoEM, obtaining maps ranging from 2.37 to 2.9 Å.
This work was done by Ilana Nodelman (Bowman lab) and Heather Folkwein (Armache lab)
Thank you to everyone involved. It was a fun joint-venture!
This work highlights a delicate balance between nucleosome acidic patch interactors critical for normal cellular functions and dysregulated in disease
Honored to be named the Laura and Isaac Perlmutter Endowed Chair in Biochemistry at NYU Grossman School of Medicine. Grateful to my mentors, lab members, and the NYU community—and most of all, to my amazing family for their constant support!
