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#single insert transformation. The problem of multiple inserts lies in your T-DNA 😳 www.biorxiv.org/content/10.6...
16h
Agrobacterium-mediated transformation is the dominant method for plant transgenesis, yet it frequently produces multi-copy, structurally complex T-DNA insertions associated with transgene silencing, u...
www.biorxiv.org
Rational design of T-DNA vectors enables predictable, single-copy integration in Arabidopsis thaliana
Ghent Rooting-lab/Steffen Vanneste