Running and maintaining cryoTEMs for external service provision @EMBL Imaging Centre. Mainly SPA but also cryoET. SerialEM fan!
Simon Fromm
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Excited to share our work uncovering SNOR, a ribosome-associated factor that promotes translation restart after dormancy published in Nature!! This was, as usual, a wonderful collaborative effort between our group and the Mattei Lab @simonemattei.bsky.social @embl.org www.nature.com/articles/s41...
A big thank you to all the cryo-EM users at @embl.org for testing and adopting LowDoseWizard — your feedback has been invaluable! 🙏
But does it actually work? 👇
At least with Apoferritin 🔎 reconstructions at 1.62 Å on a Glacios and 1.09 Å on a Titan Krios. Raw data available on EMPIAR:
Krios: www.ebi.ac.uk/empiar/EMPIA...
Glacios: www.ebi.ac.uk/empiar/EMPIA...
The workflow is fully open and comes with detailed documentation to get you started on your TEM 📖
git.embl.org/grp-ic/lowdo...
New preprint🧊🔬Introducing LowDoseWizard—a guided SerialEM workflow for rapid, standardised cryo-TEM setup!
Setting up low-dose can be slow & daunting, especially for new users. LowDoseWizard automates optics, camera, and alignment steps from minimal input — full setup in <15 min.
#cryoEM #SerialEM
