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We are scientists interested in understanding the basics of how a cell, the building block of our body, works. @biozentrum @UniBasel_en #cellbio #Biozentrum #RNA #organelles #membranes #aging #stress #proteostasis #signaling

What if you could cut out sub cellular structures and measure the proteome? SPEx: Compartment-Resolved Proteomics via Expansion Microscopy–Guided Microdissection www.biorxiv.org/content/10.6... awesome work by Curdin Franziscus, @imcf-lex.bsky.social, @biehli.bsky.social, Alexander Schmidt!

1 week left to submit an abstract to be considered for a talk at the FASEB GTPase conference: events.faseb.org/event/Small-...

Exciting preprint from @franbottanelli.bsky.social and @annespang.bsky.social! ARF-1 regulates FERARI-mediated endosomal kiss-and-run recycling by controlling selective cargo loading into vesicles. ARF-1 Coordinates Cargo Sorting at FERARI Endosomal Recycling Hubs www.biorxiv.org/content/10.6...

How to determine the proteome of cellular compartments? We established a method to do just that, published n @eLife.bsky.social: SPEx: Compartment-Resolved Proteomics via Expansion Microscopy–Guided Microdissection doi.org/10.7554/eLif...

Spang Lab's snowshoeing and sledging event. What a gorgeous day in amazing company!

The small GTPase Arf1 regulates TORC1 in two different ways! Great work by Ludovic Enkler and a great collaboration with @mihaelazavolan.bsky.social . Check out our preprint! www.biorxiv.org/content/10.6...

Check out our newest findings on how #lysosomes are generated from #endolysosomes. Great work from @samit2104.bsky.social ! @biozentrum.unibas.ch , @unibas.ch #cellbio #cell #organelles Ca2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites www.biorxiv.org/content/10.6...

Check out our new paper on mRNA levels + P-body condensate properties. Congrats to Debdatto Mookherjee and Madeleine Rommel, super collaboration with @mariahondele.bsky.social ,FerdinandWeidner, Matej Siketanc Non-translated mRNA levels determine P-body properties www.biorxiv.org/content/10.6...

Lysosomes are essential in maintaining cellular health. Endocytic lysosome reformation (ELR) regenerates functional lysosomes following degradation of endocytic cargo, yet the mechanisms driving this process remain largely unknown. Here, we define the molecular machinery underlying ELR. We find that unlike autophagic lysosome reformation (ALR), ELR proceeds independently of mTOR and dynamin 2, but requires the mitochondrial fission GTPase DRP1. DRP1 mediates scission of endolysosomal tubules at contact sites with the endoplasmic reticulum (ER) and mitochondria. Disruption of DRP1 function or ER endolysosome contact results in elongated tubules, indicating defective lysosome reformation. Moreover, mitochondrial activity is essential for tubule initiation, and Ca2+ transfer from endolysosomes to mitochondria is crucial for ELR onset. Our findings reveal a dual role for mitochondria in ELR: first in ELR initiation and second in DRP1-dependent tubule fission at ER-mitochondria-endolysosome tripartite contact sites, uncovering the previously unappreciated role of mitochondria in endolysosome remodeling and fission. ### Competing Interest Statement The authors have declared no competing interest. Swiss National Science Foundation, 320030-231859, 310030-197779 University of Basel, https://ror.org/02s6k3f65

The cell biology community mourns the loss of Catherine Rabouille, an exceptional scientist whose determination, innovation, and fearless engagement with ideas reshaped how we think about cellular organization. #InMemoriam from @adamgrieve.bsky.social and colleagues: rupress.org/jcb/article/...

Journal of Cell Biology

TORC1 spatial segregation and hysteresis are regulated by the small GTPase Arf1 https://www.biorxiv.org/content/10.64898/2026.04.29.721593v1