Oncologist-Scientist @ Princess Margaret Cancer Centre & University of Toronto 🇨🇦
Transcriptional plasticity, functional clonal heterogeneity, cancer stem cells 🧬🔬🧫
lvnguyenlab.ca
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Long V. Nguyen
Online now: The tumor microenvironment of 14,837 breast cancers is associated with clinical outcome independently of genomic subtypes
Tu et al. present a systematic analysis of the tumor microenvironment across 14,837 breast cancers, identifying distinct cellular patterns that predict disease-specific survival independently of genomic and intrinsic subtypes. Characterizing microenvironment composition may provide critical prognostic markers that can complement current molecular classification for treatment selection and preventing recurrence.
🧬 Using single‑cell barcoding and scRNA‑seq across breast cancer xenografts, we show that chemotherapy rapidly rewires the tumor cellular clonal landscape. Treatment selects for previously rare, resistant cell clones, with distinct responses across cell states and subtypes.
My poor laptop wishes I learned how to use the cluster earlier, but it’s sacrifice was not in vain! We learned some important things about the breast cancer tumor microenvironment, described in our paper published today:
www.cell.com/cell-reports...
Here's the 🧵
🔍 Stress‑adaptive, slow‑cycling “persister” programs—marked by DUSP1 and KLF4—emerge in ER+/HER2− models, revealing new insights into chemotherapy tolerance.
🔬 Tracking resistance at single‑clone resolution changes how we think about treatment failure.
lvnguyenlab.ca/2026/04/06/p...
🚨 Pre-print alert! Kronheim S et al. Chemotherapy reshapes cellular clonal landscape via persister programs in breast cancer xenografts: doi.org/10.64898/202...
In collaboration with @carloscaldas1960.bsky.social & @fgaiti.bsky.social
#CancerResearch #SingleCell #Chemotherapy #TumorEvolution
Long V. Nguyen
Important protocol from @nguyenlab.bsky.social that details method used in our paper published recently @cp-cellreports.bsky.social: www.cell.com/cell-reports...
www.sciencedirect.com/science/arti...
Kevin Tu
Long V. Nguyen
Long V. Nguyen
Most #cancer cells aren’t able to propagate tumours—but a rare few can.
Using genetic barcodes, a team @pmresearch-uhn.bsky.social tracked these rare clones—revealing how they evolve, adapt, and resist treatment. A step toward more targeted cancer therapies.
🔗: www.uhnresearch.ca/news/rare-ca...
