Thanks and congrats to lead authors Irfana Saleem and Thomas Miller and the other co-authors for their incredible work on this project.
Tony Mustoe
This additional data enables direct discovery and annotation of tertiary motifs and protein binding sites. In two examples in our paper, we use msDMS-MaP uncover new relationships between rRNA tertiary folding and ribosome assembly, and new protein binding sites on the human 7SK RNA in cells.
msDMS-MaP is a simple extension of standard DMS probing experiments that helps solve this limitation. We developed a new strategy that can simultaneously measure multiple types of DMS modifications that report on RNA secondary structure (N1/N3) AND higher-order structure (N7-G).
As many know, DMS probing experiments are amazing at mapping RNA secondary structure, but are less capable at identifying higher-order tertiary structures and protein binding sites. Because higher-order structures are often the most functionally interesting, this represents a significant limitation.
Pleased to share our latest paper, out today in Molecular Cell: “Multi-site DMS probing reveals higher-order structure of RNA-protein complexes in living cells”
www.cell.com/molecular-ce...
We are very excited about the long-term potential of msDMS-MaP to improve understanding of RNA biology and enable applications such as RNA targeted drug discovery. The experiments are remarkably easy, so if you are using DMS, I encourage you try out msDMS-MaP yourself!
Tony Mustoe
Tony Mustoe
Tony Mustoe
Check out the new method that reveals structures of #RNA-protein complexes in living cells. @amustoe.bsky.social @bcmhouston.bsky.social @cp-molcell.bsky.social www.eurekalert.org/news-release...
Think of #RNA in #3D. And with this new method from the @amustoe.bsky.social lab et al researchers can reveal RNA-protein interactions in #livingCells. @cp-molcell.bsky.social @bcmhouston.bsky.social blogs.bcm.edu/2026/04/30/f...
Saleem et al. introduce multi-site DMS-MaP (msDMS-MaP), a chemical probing strategy
that permits simultaneous mapping of RNA secondary structures and typically concealed
tertiary structures and protei...
