I'll be writing a few posts about new features in Micro-Manager. If you are interested in those, please follow @micro-manager.bsky.social.
HiCaRI: A StayGold-based calcium ion indicator by Robert E. Campbell and team: www.biorxiv.org/content/10.6...
βUnits of the NIH are privately directing grantees to request permission in advance for any co-authorship with a scholar affiliated with a foreign institution, even if all the work was done in the United States.β
Grantees also told to remove papers with foreign authors from progress reports.
Because @tanner-fadero.bsky.social asked for it and I had tokens to spare, I built an orthogonal viewer for @micro-manager.bsky.social today.
Nico Stuurman
NIH, NASA grantees are confused and concerned amid agenciesβ piecemeal communication
Random thanks to the image.sc forum, for such a treasure trove of information on bioimage analysis.
It's always a great read!
Nico Stuurman
After 9 years, I am finally finishing the story about normalized cross-correlation (and reviving my blog). The new blog post about deep technical details on the most used image registration technique. I know y'all have been waiting. katpyxa.info/feedbacks/?p...
Finally online @natmethods.nature.com
www.nature.com/articles/s41...
This was a fun collaboration with @finkryan.bsky.social @massecklab.bsky.social @tpatriarchi.bsky.social @martinfuhrmann.bsky.social @campbell-lab.bsky.social @jangrundemann.bsky.social
π§ͺπ§ π¬π§¬
Mirror mirror on the wall who is the brightest of them all?
Brightness is a key parameter for choosing fluorescent reporters in bioimaging. As tricky to assess, we present a pipeline to compare chemigenetic labels and FPs in tissue culture and #zebrafish embryos.
www.biorxiv.org/content/10.6...
Scientific Community Image Forum is a discussion forum for scientific image software sponsored by the Center for Open Bioimage Analysis (COBA).
PinkyCaMP is a red genetically encoded calcium indicator with improved brightness and photostability. Derived from mScarlet, it does not exhibit photoswitching upon blue light illumination and is ther...
A high-affinity split-HaloTag for live-cell protein labeling. And much more.
www.nature.com/articles/s41...
Weβre hiring a scientist to work on microscopy at Calico! If youβre excited about developing and applying cutting edge microscopy to a variety of biological questions, weβd love to hear from you.
Find more information and apply here: www.calicolabs.com/careers/?gh_...
David Gaboriau
Development of Micro-Manager code has accelerated quite a bit with availability of the new generation of AI coding tools (and the need to provide functionality for microscopes in our core at UCSF). Follow these developments here: nicost.github.io/microscope-t...
Eugene Katrukha πΊπ¦
Jonas Wietek
Falk Schneider
Nature Communications - Lin and colleagues present high-affinity split-HaloTag pairs for protein tagging and multiplexed labelling. This versatile system allows protein visualisation with diverse...