Nature Reviews Bioengineering, Published online: 03 June 2026; doi:10.1038/s44222-026-00454-2Rising infertility rates have increased reliance on assisted reproductive technologies, yet many such technologies remain manual, subjective and hard to standardize. This Review surveys advances in artificial intelligence and automation technologies across the embryology lab, highlighting emerging tools, clinical opportunities and challenges, and envisioning an integrated, adaptive, artificial intelligence-driven in vitro fertilization laboratory.