The Structure of the Chemotype Determining Locus in Cannabis sativa (Keith Allen, Anthony Torres, Reginald Gaudino) doi.org/10.1002/pld3... #PlantScience @aspbofficial @wileyecology @sebiology
doi.org
Functional characterization of recombinant cannabinoid oxidocyclases. Purified proteins, secreted from sf9 cells infected with recombinant baculovirus, were functionally characterized. All assays were supplemented with CBGA precursor. Activity presented as percent of total CBGA converted was normalized against canonical CBCAS activity at 100% functionality. With CBCA as the dominant product of these enzymes, comparative analysis of activity for Clade C.4, Clade C.5, and Clade C.6 was carried out against CBCAS. In assays with purified protein from sf9 expression lower activity of CBCA production was observed in Clade C recombinant enzymes. Clade C.4 comparative activity was 26% of CBCAS, while Clade C.5 and Clade C.6 were 16% and 11%, respectively, of CBCAS.
