PI in the Institute of Molecular Biology at Academia Sinica. Studying polymicrobial interaction in bacterial community. http://www.thetinglab.com
See-Yeun Ting 陳詩允
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Combined with the high resolution cryoEM structures of two Hcp1–effector complexes, Hcp1–Tse2 and Hcp1–Tse4, and additional Hcp1–effector models, we now better understand how these very different toxins can all fit inside the same Hcp tube!
See-Yeun Ting 陳詩允
In this study, we focus on Hcp, the inner-tube protein of the T6SS and an important carrier for toxic effectors!
Using the P. aeruginosa H1-T6SS as a model, we combined competition-coupled DMS, cryo-EM, competition assays, and comparative structural analysis to tackle the long standing question!
By functionally profiling thousands of Hcp1 variants, we were able to distinguish residues required for building the secretion tube from lumen-facing residues involved in effector interaction.
Interestingly, we found that Hcp1 does not recognize them through a single conserved sequence motif. Instead, Hcp1 uses shared but dispersed interaction surfaces that are mainly polar and can tolerate substantial sequence variation.
The cryoEM structures also suggest that effectors change conformation when they are loaded into Hcp1. This may help them better fit in the tube and prevent premature toxin activity before delivery.
Excited to share our new preprint on a question that has puzzled us for a long time: How can the bacterial nano-weapon, type VI secretion system (T6SS), load and deliver so many antibacterial toxins that are completely different in structure and function?
www.biorxiv.org/content/10.6...
There are much more in the article! Take a peak if you are interested in our story! Congratulations to the first author: Po-Yin Chen and the entire team!
#Microbiology #T6SS #CryoEM #DeepMutationalScan #BacterialCompetition
Our results propose that Hcp is not simply a passive tube component! It acts as a selective but flexible cargo scaffold, allowing a conserved secretion system to transport a wide range of toxic effectors for interbacterial competition!
See-Yeun Ting 陳詩允
See-Yeun Ting 陳詩允
See-Yeun Ting 陳詩允
See-Yeun Ting 陳詩允
www.biorxiv.org
See-Yeun Ting 陳詩允
In a series of workshops last year, we discussed the term ‘synthetic community’ and its usage. It’s so loosely defined and is regularly used to define something which in my eyes isn’t a SynCom. The discussion was also touching on whether the term itself is appropriate.
See-Yeun Ting 陳詩允
See-Yeun Ting 陳詩允
Benjamin Coltman
New online! Revisiting the life cycle of temperate phages
