Fantastic collaboration across @biohub.org ! Special shout-out to Chad Liu, Alex Hillsley, Madhurya Sekhar, Cassidy Jones, Gav Sturm & Taihei Fujimori. 🙌
4/4
📣 new preprint multimodal atlas. Imaging + scRNA, 57M cells. 🧬🔬
Cells are complex dynamical systems — but most ways we measure them destroy them. We asked: how does live imaging compare to scRNA-seq, the field’s gold std?
The answer surprised us 🧵
www.biorxiv.org/content/10.6...
We profiled 1,000 gene KOs in A549 cells across three modalities:
• Fluorescence imaging (39 live + 13 fixed markers)
• Label-free phase imaging of the same live cells
• scRNA-seq via CROP-seq
>57M single cell images. >600k scRNA. 100% open.
2/n
TLDR: all modalities are super powerful at resolving cellular states. But phase imaging matches — and given enough cells, exceeds — the phenotypic resolution of both fluorescence and scRNA-seq.
This lays the foundation for live-cell profiling of phenotypic trajectories. 🚀
3/n
Time to share our organelle proteome paper
out in @cellcellpress.bsky.social: doi.org/nzwz
We created a spatial map of human cells using organelle immunoprecipitation at scale.
Locate your fav protein among the 7,600 we mapped across 19 subcellular compartments: organelles.sf.czbiohub.org/
1/3 🧪
Great news! @mariaingaramo.bsky.social 's company (Nonfiction Labs) made a remote-controlled antibody.
Its binding turns on and off with a magnet.
This is a HUGE step towards our dream of magnetically controlled drugs. Imagine a cancer drug that ONLY attacks the tumor, not the rest of your body.
