We next asked: how does KLF2 work?
Using perturb-SHARE-seq, we applied seq2PRINT and found that RUNX TFs are highly collaborative, enabling cells to access diverse regulatory programs. Additional experiments revealed that RUNX2 and KLF2 physically interact to regulate key exhaustion genes. 🧬
We developed PRINT, a computational method that identifies footprints of DNA–protein interactions from bulk and single-cell chromatin accessibility data across multiple scales of protein size.
