PhD Candidate @embl.org | European Molecular Biology Laboratory | Duss and Hennig groups
#RNA structure and dynamics | #single-molecule | #biophysics | #structuralBiology | #translation | #biochemistry
Marco Payr
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🧵 (7/7) Combining different methodologies (smFRET, NMR & reporter gene assays) we developed a framework that can now be used to study similar processes in other biological systems! 🧬
hashtag#single-molecule hashtag#FRET hashtag#RNA hashtag#translation hashtag#Biophysics
🧵 (2/7) Key Finding #2: We saw Sxl can slide along RNA between binding sites instead of just binding and falling off. This helps it stay attached longer. 🏄♂️
🧵 (1/7) Key Finding #1: We discovered Sex-lethal (Sxl) alone, despite its high affinity, transiently samples its target sites
🧵 (3/7) Key Finding #3: RNA-bound Sxl helps recruit another protein (Unr) 500x faster! Like a molecular speedway for protein assembly. 🏃♂️💨
🧵 (4/7) Key Finding #4: A third protein (Hrp48) acts as a molecular "doorstop" - preventing RNA from displacing Sxl and Unr. 🚪
🧵 (6/7) Why this matters: Understanding these molecular mechanisms helps us grasp how cells regulate their genes through different complex assembly mechanisms.
🧵 (5/7) These mechanisms work together to achieve tight translation repression. And it’s the first time seeing this complex assembly in real-time!
In a collaboration between the @hennig-lab.bsky.social and @olivierduss.bsky.social labs we revealed how proteins work together to control gene expression through mRNA translation repression! Using single-molecule microscopy, we watched individual molecules in action. doi.org/10.1101/2025... 🧵
Marco Payr
We have 3 openings for ambitious postdocs or PhD students in our #CDlab - for exciting single-molecule biophysics research on nuclear pores, peroxisomes, or archaeal divisomes.
Check it out and apply: ceesdekkerlab.nl/come-join-us/
RT=nice!
Marco Payr
Marco Payr
Marco Payr
Video
🔔 Proud to share the preprint of my PhD work in the Petridou group @nicolettapetridou.bsky.social @embl.org ✨
“A closed feedback between tissue phase transitions and morphogen gradients drives patterning dynamics” 🐟 🔁 📶
🔗 www.biorxiv.org/content/10.1...
#devbio #biophysics
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