Post 2/3
Key highlights:
• ⚙️ Dual, allele-specific integration into distinct genomic alleles
• 🎯 High efficiency & specificity with strong enrichment after selection
• 🧬 Programmable gene expression via promoter & gene design
• 🔁 Scalable genome engineering with orthogonal inducible systems
💸 Stem cell culture that’s ~95% cheaper and weekend-free: a win-win for PIs and trainees!
Our revised manuscript is online at ORE: open-research-europe.ec.europa.eu/articles/4-1...
Benchmarked across cell lines & differentiation protocols, including scRNA-seq — with lessons for #cultivatedmeat too!
⭕️ The STRAIGHT-IN dual plasmids are now available on Addgene! 🧬
@addgene.bsky.social
A full STRAIGHT-IN kit with 20+ plasmids, including everything needed to set up and run the platform, is coming soon 🔥
Stay tuned 🚀
Our review, "Revitalizing the heart: strategies and tools for cardiomyocyte regeneration post-myocardial infarction." doi.org/10.1038/s415..., has just been published in npj Regenerative Medicine!
Post 3/3
Direct application using STRAIGHT-IN Dual is also out at @science.org
We show how gene syntax shapes gene expression through DNA supercoiling, revealing a new layer of regulation. We built homozygous hiPSC lines with identical payloads at CLYBL & AAVS1
Link: www.science.org/doi/10.1126/...
With Christmas just around the corner, it’s time for the #FluorescenceFriday post🎄!
This week, we’re featuring a hiPSC reporter line engineered to express 2 fluorescent proteins—located in the nuclei and actin filaments of #hiPSCs, generated using STRAIGHT-IN Dual🧪🔬
@benjaminbjohnson.bsky.social?
Post 1/3 🧵
Our latest work is out at @natbiomedeng.nature.com!🚀
We present STRAIGHT-IN Dual — a platform for precise, allele-specific integration of two large DNA payloads into human iPSCs, enabling more sophisticated control of complex genetic programs.
Link📝: www.nature.com/articles/s41...
👇
Created a second stem cell biology starter pack, as the first one was full.
Let me know if you would like to be added!
go.bsky.app/UG3eYwU
There are so many #morphogenesis researchers here!
My first starter pack is full!
Here is Part 2: go.bsky.app/K6YYKeC
#devbio #cellbio #science
Tell me if I should add you but try to make sure, that you're not already in Part 1.
I cannot cross-reference everyone 😅
Part 1: go.bsky.app/RzDD9or
How to fix a broken #heart 💔->❤️
#Proteoglycan rich #ECMatrix scar tissue is laid by migratory cells after a cardiac scratch wound with #hiPSC #Cardiomyocytes.
Happy #Fluorescencefriday #BlueSky & #CardioSky!
@ablanchasensio.bsky.social 👀
#Science 💥🧪 #Microscopy 🔬
Need a solution for multi-transgene integration? Check out the STRAIGHT-IN Dual platform! Thanks to the Galloway and Davis Labs for sharing the plasmids: www.addgene.org/browse/artic...
npj Regenerative Medicine - Revitalizing the heart: strategies and tools for cardiomyocyte regeneration post-myocardial infarction
Post 1/3 🧵
Our latest work is out at @natbiomedeng.nature.com!🚀
We present STRAIGHT-IN Dual — a platform for precise, allele-specific integration of two large DNA payloads into human iPSCs, enabling more sophisticated control of complex genetic programs.
Link📝: www.nature.com/articles/s41...
👇
