Re-upping for morning phage phriends. Hope you will find Brenna & Patrick's method useful!
Here they are, with some other lab members, on a rock.
They are rock stars.
Jesse Shapiro
Cheap and easy method for nanopore sequencing phage DNA directly from plaques.
Proud of lab members Brenna and Patrick for doing this work *with absolutely no help from me* !!
www.nature.com/articles/s41...
Scientific Reports - Same-day tagmentation PCR-based whole genome sequencing of bacteriophage genomes from a single plaque without DNA extraction