(RCM) Bioanalysis of Ametryn by UHPLC‐MS3 Coupled With Multiple Fragmentation to Decrease Interference and Enhance Sensitivity: ABSTRACT
Rationale
Ametryn poses significant risks to aquatic ecosystems, but conventional MRM methods suffer from matrix… #RapidCommunMassSpectrom #MassSpecRSS
ABSTRACT
Rationale
Ametryn poses significant risks to aquatic ecosystems, but conventional MRM methods suffer from matrix interference and limited sensitivity. To overcome these limitations, we established a more selective and sensitive UHPLC-MS3 approach that effectively suppresses background noise and enhances signal-to-noise ratio, enabling reliable quantification of ametryn in zebrafish tissues.
Methods
A novel UHPLC-MS3 method with electrospray ionization was developed. Quantification employed the MS3 transition m/z 228.0 → 186.1 → 158.0. Five zebrafish tissues (liver, muscle, brain, intestine, gill) were analyzed following sample preparation. Fast chromatographic separation was achieved in 2.8 min.
Results
Compared with MRM, the MS3 method substantially reduced matrix interference, enhanced signal intensity from 6.4 × 104 to 1.6 × 107 cps, and increased the signal-to-noise ratio from 6.3 to 45. The assay exhibited excellent linearity (10–1000 pg/mL), and validation results for accuracy, precision, recovery, matrix effects, and stability all met FDA bioanalytical requirements.
Conclusions
This study presents the first application of UHPLC-MS3 for quantitative analysis of ametryn in multiple zebrafish tissues, providing a reliable high-throughput platform for residue monitoring and mechanistic toxicology research.
