Co-led by extraordinary trainees Dr. Bo Cai and Andrew Xue, with collaborators Rogelio Hernández-López and postdoc Qian Xue. Xiaojie Qiu and his postdoc Nianping Liu applied Dynamo to analyze CAR T state transitions. Thanks to NSF, CIRM, and Biohub for funding.
www.biorxiv.org/cgi/content/...
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(The setting is pretty nice too…)
Overexpressing CTSW in CAR T cells → larger fraction of stem-like memory phenotype (CD62L+CD45RA+), increased proliferation, and dramatically better tumor killing over repeated rounds of antigen challenge!
Why sequence compartment-specific RNAs instead of whole-cell transcriptomes?
Because RNA localization controls splicing, translation, and degradation. Changes in RNA localization (independent of abundance changes) are undetectable by conventional scRNA-seq.