🚨 New preprint: GATO-seq did it again! ELOF1 is the missing promoter-proximal factor that confers RNA Pol II resistance to TFIIF. Including ELOF1, DSIF, and NELF in GATO-seq reactions recapitulates promoter-proximal pausing in vitro at physiological conditions for the 1st time.
tinyurl.com/ELOF1
📣 Preprint alert! We developed a method to analyze concurrent interactions between multiple chromatin regions at single alleles at sub-nucleosome resolution (multi-way Micro-Capture-C, mwMCC) & used this to study structural synergy within super-enhancers. 1/14
www.biorxiv.org/content/10.6...
Out today - structure of the human HIRA histone chaperone complex bound to nucleosomes. Ever wondered how nucleosomes are assembled in the wake of transcription? It takes a 'hulk of a protein complex'. Work by the amazing Wei Tian weetian558.bsky.social. www.biorxiv.org/content/10.6... 🧵
Congratulations to Della Syau and the Farnung Lab @lucas.farnunglab.com on this outstanding work!
⭐ #NARBreakthrough! ⭐
New #cryo-EM study reveals how #transcription factor IWS1 promotes #RNApolII #elongation. Its C-terminus contains #modularmotifs that bind elongation components, stabilizing the complex. IWS1 also competes with #RECQL5 for Pol II binding.
📖 Read here: doi.org/10.1093/nar/...
This work also represents the first structure of a histone reader (LEDGF's PWWP domain) bound to an actively transcribed nucleosome.
Open q to the #cryoem crowd - what’s your fav NN/ML picker for hard SPA targets?
We use topaz with good results, but there have been a bazillion new pickers over the last years…
It is often hard to tell from the paper on each picker, as the training data matters a lot for topaz at least..
Excited that Della Syau's PhD work is now available as a NAR Breakthrough article: academic.oup.com/nar/article/....
Della's work clarifies how IWS1 engages the transcription elongation complex, stimulates transcription, and controls association of other factors such as RECQL5.
One, perhaps two, of our postdocs are moving on to faculty positions this year (more news soon!). While they're truly irreplaceable, it does mean that we have openings for new members to join our team studying #cilia. If interested, please apply: academicpositions.harvard.edu/postings/15526